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Characterizing Interactions of Tolfenpyrad with Antibiotics Against Francisella novicida: Synergy, Antagonism, 
and Mechanistic Insights

frans.jfif

Summer 2024 - Present

Research Conducted under Dr. Aria Eshraghi

Department of Infectious Diseases & Immunology, University of Florida

Research Description:

Francisella tularensis is a highly virulent Gram-negative bacterial pathogen that can be transmitted via aerosols and arthropod bites. As one of the most infectious pathogens known, as few as 10 viable bacteria can cause a deadly disease. This highlights both its potential use as a bioweapon and the urgency to develop new strategies to safeguard against antimicrobial resistance. Thus, it is essential to innovate novel and improve existing treatments against Francisella. We recently identified a pesticide, tolfenpyrad, that potently blocks Francisella growth; however, the mechanism that tolfenpyrad employs to disrupt Francisella growth is unknown. This research aims to shed light on tolfenpyrad's mechanism of action.

Project Responsibilities:

  • Conduct plate-based assays with combinations of tolfenpyrad and characterized antibiotics to assess synergism and antagonism.

  • Utilize flow cytometry to assess fluorescence of tetracycline when in combination with tolfenpyrad.

  • Perform basic lab duties, such as producing agar plates and proper biohazardous waste removal.

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